Broad-Spectrum Detection of H5 Subtype Influenza A Viruses with a New Fluorescent Immunochromatography System
Identifieur interne : 000909 ( Main/Exploration ); précédent : 000908; suivant : 000910Broad-Spectrum Detection of H5 Subtype Influenza A Viruses with a New Fluorescent Immunochromatography System
Auteurs : Akira Sakurai [Japon] ; Katsuyoshi Takayama [Japon] ; Namiko Nomura [Japon] ; Tsubasa Munakata [Japon] ; Naoki Yamamoto [Japon] ; Tsuruki Tamura [Japon] ; Jitsuho Yamada [Japon] ; Masako Hashimoto [Japon] ; Kazuhiko Kuwahara [Japon] ; Yoshihiro Sakoda [Japon] ; Yoshihiko Suda [Japon] ; Yukuharu Kobayashi [Japon] ; Nobuo Sakaguchi [Japon] ; Hiroshi Kida [Japon] ; Michinori Kohara [Japon] ; Futoshi Shibasaki [Japon]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Immunochromatography (IC) is an antigen-detection assay that plays an important role in the rapid diagnosis of influenza virus because the protocol is short time and easy to use. Despite the usability of IC, the sensitivity is approximately 103 pfu per reaction. In addition, antigen-antibody interaction-based method cannot be used for the detection of influenza viruses with major antigenic change. In this study, we established the use of fluorescent immunochromatography (FLIC) to detect a broad spectrum of H5 subtype influenza A viruses. This method has improved sensitivity 10–100 fold higher than traditional IC because of the use of fluorescent conjugated beads. Our Type-E FLIC kit detected all of the H5 subtype influenza viruses that were examined, as well as recombinant hemagglutinin (HA) proteins (rHAs) belonging to the Eurasian H5 subtype viruses and the Type-N diagnosed North American H5 subtype influenza A viruses. Thus, this kit has the improved potential to detect H5 subtype influenza viruses of different clades with both Type-E and Type-N FLIC kits. Compared with PCR-based diagnosis, FLIC has a strong advantage in usability, because the sample preparation required for FLIC is only mix-and-drop without any additional steps such as RNA extraction. Our results can provide new strategies against the spread and transmission of HPAI H5N1 viruses in birds and mammals including humans.
Url:
DOI: 10.1371/journal.pone.0076753
PubMed: 24223117
PubMed Central: 3819354
Affiliations:
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Usa-City, Oita, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>ADTEC Co., LTD. 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Usa-City, Oita, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>ADTEC Co., LTD. Usa-City, Oita</wicri:regionArea><wicri:noRegion>Oita</wicri:noRegion></affiliation></author><author><name sortKey="Nomura, Namiko" sort="Nomura, Namiko" uniqKey="Nomura N" first="Namiko" last="Nomura">Namiko Nomura</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Munakata, Tsubasa" sort="Munakata, Tsubasa" uniqKey="Munakata T" first="Tsubasa" last="Munakata">Tsubasa Munakata</name><affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo</wicri:regionArea><wicri:noRegion>Sapporo</wicri:noRegion></affiliation></author><author><name sortKey="Tamura, Tsuruki" sort="Tamura, Tsuruki" uniqKey="Tamura T" first="Tsuruki" last="Tamura">Tsuruki Tamura</name><affiliation wicri:level="3"><nlm:aff id="aff5"><addr-line>Konica Minolta, Inc., Hino-shi, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Konica Minolta, Inc., Hino-shi, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yamada, Jitsuho" sort="Yamada, Jitsuho" uniqKey="Yamada J" first="Jitsuho" last="Yamada">Jitsuho Yamada</name><affiliation wicri:level="3"><nlm:aff id="aff5"><addr-line>Konica Minolta, Inc., Hino-shi, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Konica Minolta, Inc., Hino-shi, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Hashimoto, Masako" sort="Hashimoto, Masako" uniqKey="Hashimoto M" first="Masako" last="Hashimoto">Masako Hashimoto</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kuwahara, Kazuhiko" sort="Kuwahara, Kazuhiko" uniqKey="Kuwahara K" first="Kazuhiko" last="Kuwahara">Kazuhiko Kuwahara</name><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Department of Immunology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Chuo-ku, Kumamoto, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Chuo-ku, Kumamoto</wicri:regionArea><wicri:noRegion>Kumamoto</wicri:noRegion></affiliation></author><author><name sortKey="Sakoda, Yoshihiro" sort="Sakoda, Yoshihiro" uniqKey="Sakoda Y" first="Yoshihiro" last="Sakoda">Yoshihiro Sakoda</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo</wicri:regionArea><wicri:noRegion>Sapporo</wicri:noRegion></affiliation></author><author><name sortKey="Suda, Yoshihiko" sort="Suda, Yoshihiko" uniqKey="Suda Y" first="Yoshihiko" last="Suda">Yoshihiko Suda</name><affiliation wicri:level="3"><nlm:aff id="aff5"><addr-line>Konica Minolta, Inc., Hino-shi, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Konica Minolta, Inc., Hino-shi, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kobayashi, Yukuharu" sort="Kobayashi, Yukuharu" uniqKey="Kobayashi Y" first="Yukuharu" last="Kobayashi">Yukuharu Kobayashi</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>ADTEC Co., LTD. Usa-City, Oita, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>ADTEC Co., LTD. Usa-City, Oita</wicri:regionArea><wicri:noRegion>Oita</wicri:noRegion></affiliation></author><author><name sortKey="Sakaguchi, Nobuo" sort="Sakaguchi, Nobuo" uniqKey="Sakaguchi N" first="Nobuo" last="Sakaguchi">Nobuo Sakaguchi</name><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Department of Immunology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Chuo-ku, Kumamoto, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Chuo-ku, Kumamoto</wicri:regionArea><wicri:noRegion>Kumamoto</wicri:noRegion></affiliation></author><author><name sortKey="Kida, Hiroshi" sort="Kida, Hiroshi" uniqKey="Kida H" first="Hiroshi" last="Kida">Hiroshi Kida</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo</wicri:regionArea><wicri:noRegion>Sapporo</wicri:noRegion></affiliation></author><author><name sortKey="Kohara, Michinori" sort="Kohara, Michinori" uniqKey="Kohara M" first="Michinori" last="Kohara">Michinori Kohara</name><affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Shibasaki, Futoshi" sort="Shibasaki, Futoshi" uniqKey="Shibasaki F" first="Futoshi" last="Shibasaki">Futoshi Shibasaki</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Immunochromatography (IC) is an antigen-detection assay that plays an important role in the rapid diagnosis of influenza virus because the protocol is short time and easy to use. Despite the usability of IC, the sensitivity is approximately 10<sup>3</sup> pfu per reaction. In addition, antigen-antibody interaction-based method cannot be used for the detection of influenza viruses with major antigenic change. In this study, we established the use of fluorescent immunochromatography (FLIC) to detect a broad spectrum of H5 subtype influenza A viruses. This method has improved sensitivity 10–100 fold higher than traditional IC because of the use of fluorescent conjugated beads. Our Type-E FLIC kit detected all of the H5 subtype influenza viruses that were examined, as well as recombinant hemagglutinin (HA) proteins (rHAs) belonging to the Eurasian H5 subtype viruses and the Type-N diagnosed North American H5 subtype influenza A viruses. Thus, this kit has the improved potential to detect H5 subtype influenza viruses of different clades with both Type-E and Type-N FLIC kits. Compared with PCR-based diagnosis, FLIC has a strong advantage in usability, because the sample preparation required for FLIC is only mix-and-drop without any additional steps such as RNA extraction. Our results can provide new strategies against the spread and transmission of HPAI H5N1 viruses in birds and mammals including humans.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Claas, Ec" uniqKey="Claas E">EC Claas</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Van Beek, R" uniqKey="Van Beek R">R van Beek</name></author><author><name sortKey="De Jong, Jc" uniqKey="De Jong J">JC De Jong</name></author><author><name sortKey="Rimmelzwaan, Gf" uniqKey="Rimmelzwaan G">GF Rimmelzwaan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Jong, Jc" uniqKey="De Jong J">JC de Jong</name></author><author><name sortKey="Claas, Ec" uniqKey="Claas E">EC Claas</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG 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first="Michinori" last="Kohara">Michinori Kohara</name><name sortKey="Kuwahara, Kazuhiko" sort="Kuwahara, Kazuhiko" uniqKey="Kuwahara K" first="Kazuhiko" last="Kuwahara">Kazuhiko Kuwahara</name><name sortKey="Munakata, Tsubasa" sort="Munakata, Tsubasa" uniqKey="Munakata T" first="Tsubasa" last="Munakata">Tsubasa Munakata</name><name sortKey="Nomura, Namiko" sort="Nomura, Namiko" uniqKey="Nomura N" first="Namiko" last="Nomura">Namiko Nomura</name><name sortKey="Sakaguchi, Nobuo" sort="Sakaguchi, Nobuo" uniqKey="Sakaguchi N" first="Nobuo" last="Sakaguchi">Nobuo Sakaguchi</name><name sortKey="Sakoda, Yoshihiro" sort="Sakoda, Yoshihiro" uniqKey="Sakoda Y" first="Yoshihiro" last="Sakoda">Yoshihiro Sakoda</name><name sortKey="Shibasaki, Futoshi" sort="Shibasaki, Futoshi" uniqKey="Shibasaki F" first="Futoshi" last="Shibasaki">Futoshi Shibasaki</name><name sortKey="Suda, Yoshihiko" sort="Suda, Yoshihiko" uniqKey="Suda Y" first="Yoshihiko" last="Suda">Yoshihiko Suda</name><name sortKey="Takayama, Katsuyoshi" sort="Takayama, Katsuyoshi" uniqKey="Takayama K" first="Katsuyoshi" last="Takayama">Katsuyoshi Takayama</name><name sortKey="Tamura, Tsuruki" sort="Tamura, Tsuruki" uniqKey="Tamura T" first="Tsuruki" last="Tamura">Tsuruki Tamura</name><name sortKey="Yamada, Jitsuho" sort="Yamada, Jitsuho" uniqKey="Yamada J" first="Jitsuho" last="Yamada">Jitsuho Yamada</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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